Comparative genomic analysis of Mycobacterium immunogenum strain CD11_6, a new potential vaccine strain against Mycobacterium tuberculosis

Authors Affiliation(s)

  • 1Department of Biophysics, Panjab University, Chandigarh, INDIA
  • 2Immunology Laboratory, CSIR-Institute of Microbial Technology, Chandigarh, INDIA
  • 3Microbial Type Culture Collection and Gene bank (MTCC), CSIR-Institute of Microbial Technology, Chandigarh, INDIA
  • 4Department of Gastroenterology, Post Graduate Institute of Medical Education and Research, Chandigarh, INDIA
  • 5Department of Endocrinology, Post Graduate Institute of Medical Education and Research, Chandigarh, INDIA

Can J Biotech, Volume 1 Special Issue,  Page 131,  DOI: https://doi.org/10.24870/cjb.2017-a117

*Corresponding authors: bhadadask@rediffmail.com, javed@imtech.res.in, mayil@imtech.res.in

Abstract

Mycobacterium tuberculosis (Mtb) infection is a growing challenge to the scientific world due to drug resistance. In this study, we predicted safety, efficacy and the molecular basis for the potential of strain Mycobacterium immunognum (Mi) as a vaccine strain against Mtb that in some preliminary experiments had reduced the bacterial counts of Mtb in infected organs of mice during immunization studies along with generation of memory CD4 T cells and CD8 T cells. MI strain CD11_6 was an isolate from duodenal mucosa of a celiac disease patient that was characterized and sequenced. Rapid Annotation using Subsystem Technology (RAST) server was used to annotate, and identify the virulence determinant genes and other features that make it a potential vaccine candidate. Genome of Mtb strain H37Rv and its vaccine strain Mycobacterium bovis (Mb) AFF2122/97 was also retrieved from genome database of NCBI and compared with Mi. Virulence determinant genes of Mi were mapped and compared with virulent Mtb strain H37Rv and strain Mycobacterium bovis (Mb) AFF2122/97. This comparative analysis revealed that Mi is less virulent as compared to Mb and Mtb whereas Mi contains comparable number of genes coding for antigenic surface membrane proteins, membrane transport proteins and cytosolic proteins that predicts its probable vaccination attributes against Mtb. Further, sequence alignment results and exploring predicted proteome of the strain CD11_6 also indicates that it has potential candidate vaccine peptides belonging to membrane proteins of Mtb

The study signifies that Mi strain CD11_6 has sufficient antigenic repertoire that might have led to activate memory T cells against Mtb and causing its eradication. Our further work on this line to validate the role of reported surface membrane proteins may help to know about molecular basis for action of Mi that will improve the present vaccination strategies against Mtb.