Effect of Lead acetate on oxidative stress and antioxidant defence system of Bacillus subtilis and plasmid (pBSIISK) isolated from DH5α

Authors Affiliation(s)

  • 11Department of Zoology, Ravenshaw University, Cuttack, Odisha, INDIA
  • 2Department of Zoology, N.C. College, Jajpur, Odisha, INDIA
  • 3Department of Biotechnology, Ravenshaw University, Cuttack, Odisha, INDIA

Can J Biotech, Volume 1 Special Issue,  Page 154,  DOI: https://doi.org/10.24870/cjb.2017-a140

Presenting author: smitapatri04@gmail.com; *Corresponding author: jenasrikanta@yahoo.co.in


Environmental contamination by heavy metals has been one of the major concerns for ecological and public health. Although some heavy metals are required for metabolic processes, but their excessive accumulation in living organisms is always detrimental. High concentration of lead affects all living organisms including soil flora, fauna and microorganisms. Presence of such heavy metals in environment could certainly cause the decrease in the community diversity. This study was aimed to investigate the effect of lead acetate on growth and antioxidant defence system of Bacillus subtilis in dose (0, 0.125, 0.25 and 0.5 mM) and time (6, 12, and 24 h) dependent manner, and also asses its deleterious effects on plasmid-pBSIISK isolated from DH5α strain. The results indicate that the cell number was declined significantly with increase in concentration of the heavy metal at different time of their growth phase. Lipid peroxidation (LPx) and reduced glutathione (GSH) levels were significantly enhanced in response to lead acetate, whereas the activities of antioxidant enzymes, superoxide dismutase (SOD) and catalase (CAT) were decreased in presence of lead acetate. Glutathione S-transferase (GST) activity was increased at 6 h and 12 h, but decreased at 24 h in response to lead acetate. In vitro study indicates that lead acetate potentially damage the plasmid (pBSIISK) isolated from DH5α strain.


  1. Halliwell, B. and Gutterige, J.M.C. (2007) Free Radical in Biology and Medicine. Oxford University press Inc, New York.
  2. Valverde, M., Trejo, C. and Rojas, E. (2001) Is the capacity of lead acetate and cadmium chloride to induce genotoxic damagedue to direct DNA-metal interaction? Mutagenesis 16: 265-270.  
  3. Behera, M., Dandapat, J. and Rath, C.C. (2014) Effect of heavy metals on growth response and antioxidant defense protection in Bacillus cereus. J Basic Microbiol 54: 1201-1209. Crossref