Molecular typing of antibiotic resistant bacteria isolated and identified as ESBL producers from polluted water reservoirs

  1. Priti P. Yewale1,
  2. Aishwarya Aishwarya1,
  3. Prajakta A. Hatekar1,
  4. A. Mandal2,
  5. N. N Nawani1*,
  6. J Jass3

Authors Affiliation(s)

  • 1Microbial Diversity Research Centre, Dr. D. Y. Patil Biotechnology and Bioinformatics Institute, Dr. D. Y. Patil Vidyapeeth, Pune 411 033, INDIA
  • 2Systems Biology Research Centre, School of Biosciences, University of Skӧvde, SWEDEN
  • 3The Life Science Centre, School of Science and Technology, rebro University, rebro, SWEDEN

Can J Biotech, Volume 1, Special Issue, Page 117, DOI:

*Corresponding author:


Anthropogenic polluted reservoirs are the mating hub for antibiotic resistant genes and multidrug resistant bacteria (MDR). The rapid emergence of this MDR is the consequence of mutations in the genes as well as the horizontal gene transfer of mobile elements carrying the resistant genes. Current study focuses on isolation and characterization of Extended spectrum β-lactamase (ESBL) producers from diverse water resources of Pune city and to understand the genetic modifications responsible for multidrug resistance using whole genome sequencing (Next Generation sequencing-Illumina sequencing). The identified isolates were Pseudomonas aeruginosa, Escherichia coli, Enterobacter cloacae and Stenotrophomonas maltophilia. Mechanism of resistance developed by all isolates was efflux pump as per the genes (adeL, macA, macB, ros B) identified by Comprehensive Antibiotic Resistance Database. Primary phenotypic detection of isolates as ESBL producers and AmpC hyper producers was supportive as identified genes were resistant to all antibiotics including last resorts like carbapenems, peptide antibiotics. Rapid emergence of antibiotic resistance was seen in one isolate due to presence of additional 19 antibiotic resistant genes (blaI, exo bet lactamase, PDC 9, CMY-83, mec I, etc.). The investigation alarms the deadly pollution of reservoirs due to haphazard use of antibiotics which pressurizes rapid emergence and persistence of MDR.