Evaluation of a topical herbal drug for its in-vivo immunological effect on cytokines production and antibacterial activity in bovine subclinical mastitis

  1. Mukesh Kher1,
  2. Vaibhav Bhatt1,
  3. Navin Sheth1,
  4. Anju Kunjadia2,
  5. Dev Nauriyal3,
  6. Chaitanya Joshi4

Authors Affiliation(s)

  • 1Department of Pharmaceutical Sciences, Saurashtra University, Rajkot, Gujarat, INDIA
  • 2Center for interdisciplinary studies in science and technology (CISST), Sardar Patel University, Vallabh Vidyanagar, Gujarat, INDIA
  • 3Departments of Veterinary Medicine, Veterinary Science College, AAU, Anand, Gujarat, INDIA
  • 4Departments of Animal Biotechnology, Veterinary Science College, AAU, Anand, Gujarat, INDIA

Can J Biotech, Volume 1, Special Issue, Page 151, DOI: https://doi.org/10.24870/cjb.2017-a137

Presenting author: mnkher318@gmail.com


Mastitis is an inflammatory disorder caused by microorganisms. Currently antibiotics have been mainstay of mastitis therapy.However, their use is associated with cost issue and human health concern. Some herbs exert beneficial effects on bacterial pathogens through immunomodulation by influencing cytokine production. To assess the effect of herbs on cytokine profile, total bacterial load and somatic cell count in two breeds of cattle harboring subclinical mastitis. The response to treatment was evaluated by enumerating somatic cell count, total bacterial load and studying the expression of different cytokines (IL-6, IL-8, IL-12, IFN-Ɣ and TNF-α).The expression profiles were carried out using real time PCR, by collecting milk on days 0 as well as 5 and 21 post last treatment and data were analyzed using Statistical analysis system software. Pre and post treatment SCC in mastitic quarters statistically did not differ significantly, however, total bacterial load declined significantly from day 0 onwards in both the breeds. Highly significant differences (P < 0.01) were observed in all the cytokines on day 0, 5, and 21 post last treatments in both the breeds. The comparison between crossbred and Gir cattle revealed a significant difference in expression of IIL-6 and TNF-α. However, other cytokines exhibited a similar pattern of expression in both breeds, which was non-significant. The topical herbal drug exhibited antibacterial and immunomodulatory activities and thus the work supports its use as an alternative to antibiotics against subclinical udder infection in bovines.