Identification of Groundnut miRNA and their targets

  1. Mousam Kumar Ram,
  2. Koel Mukherjee,
  3. Dev Mani Pandey*

Authors Affiliation(s)

  • Department of Bioengineering, Birla Institute of Technology, Mesra, Ranchi 835215, INDIA

Can J Biotech, Volume 1, Special Issue, Page 194, DOI:

*Corresponding author:


MicroRNA (miRNA) are ~22nt small non-coding RNA molecules which play an important role in post-transcriptional gene regulation in both plants and animals. As the miRNAs are highly conserved among species, comparative genomics based homology search has played a key role in identifying new miRNAs in different species whose genomes are not yet sequenced. Arachis hypogaea (groundnut or peanut) is one such legume crop and being grown in more than 100 countries, ranks third worldwide among oilseeds produced. In India, it is the second largest in terms of production but stands first in terms of area of cultivation. Identifying miRNAs and their targets can be helpful in crop improvement. In the present study, we tried to identify new conserved miRNA from the 205442 ESTs through blast search, using previously known plant miRNAs. The non-protein coding sequences with homology showing no more than 3 mismatches were folded back to stem-loop structure. These were subsequently passed through strict filtration criteria to obtain new miRNAs belonging to different miRNA families, as well as their targets.


  1. Bansal, R.K., Gondaliya, V.K. and Shaikh, A.S. (2017) A review of the status of the groundnut production and export of India. Indian Journal of Economics and Development 13: 369-374. Crossref
  2. Zhang, B., Wang, Q., Wang, K., Pan, X., Liu, F., Guo, T., Cobb, G.P. and Anderson, T.A. (2007) Identification of cotton microRNAs and their targets. Gene 397: 2637. Crossref
  3. Prabu, G.R. and Mandal, A.K. (2010) Computational Identification of miRNAs and Their Target Genes from Expressed Sequence Tags of Tea (Camellia sinensis). Genomics Proteomics Bioinformatics 8: 113-121. Crossref