Production of a xylanase by Trichoderma harzianum (Hypocrea lixii) in solid-state fermentation and its recovery by an aqueous two-phase system

Uncategorized
  1. Ricardo Gómez-García1,
  2. Miguel A. Medina-Morales1,
  3. Raul Rodrìguez1,
  4. Beatriz Farruggia2,
  5. Guillermo Picó2,
  6. Cristóbal N. Aguilar1*

Authors Affiliation(s)

  • 1Research Group of Bioprocesses and Bioproducts (BBG-DIA), Food Research Department, School of Chemistry, Autonomous University of Coahuila, Saltillo, Coahuila, MÉXICO
  • 2Institute of Chemical and Biotechnological Processes (IPROBYQ-CONICET), National University of Rosario, Rosario, Santa Fe, ARGENTINA

Can J Biotech, Volume 2, Issue 2, Pages 108-115, DOI: https://doi.org/10.24870/cjb.2018-000122

Received: Jul 8, 2018; Revised: Aug 22, 2018; Accepted: Sep 7, 2018

Abstract

Production of xylanase enzyme by fungi strains has gained interest in the recent years due to its high productivity, high catalytic power, as well as its potential applications in different areas such as feed, food, textile, and biofuel industries. The conventional methodologies, to produce enzymes, involve complex apparatus and chemical solvents and are associated with high costs and low- yield recovery. To obtain the high-yield recovery of the enzymes, modern enzyme extraction methods are taken into account. Aqueous two-phase systems (ATPS) are an alternative separative methodology for the purification and recovery of the enzymes and other biomolecules. The advantages of ATPS are easy scale-up and extraction, volume reduction, and rapid separation. The objective of this study was to produce Trichoderma harzianum xylanase by solid-state fermentation (SSF) using corn cobs as a support/substrate and employing ATPS for its partial recovery. In this study, the results showed the ability of a microorganism to grow on the corn cobs and to produce the xylanase enzyme. Xylanolytic activity reached 7.85 U/g of corn cobs. The enzyme was efficiently concentrated by ATPS. In addition, a high purification factor (10-fold) and considerable enzyme recovery (%ER) (84%) percentage were obtained.

References

  1. Zhang, Y., Wang, L. and Chen, H. (2017) Correlations of medium physical properties and process performance in solid-state fermentation. Chem Eng Sci165: 65-73. Crossref
  2. Choi, J.M., Han, S.S. and Kim, H.S. (2015) Industrial applications of enzyme biocatalysis: Current status and future aspects. Biotechnol Adv 33: 1443-1454. Crossref
  3. Soccol, C.R., Ferreira da Costa, E.S., Junior-Letti, L.A., Grace-Karp, S., Woiciechowski, A.L., Souza- Vandenberghe, L.P. (2017) Recent developments and innovations in solid state fermentation. Biotechnol Res Innov 1: 52-71. Crossref
  4. Yusuf, M. (2017) ‘Agro-industrial waste materials and their recycled value-added applications : Review’ . In Handbook of Ecomaterials (Martinez L, Kharissova O, Kharisov B, Eds). Springer, Cham, 1-11. Crossref
  5. Robledo-Olivo, A., Aguilar, C.N. and Montañez-Sáenz, J.C. (2012) Uso del olote de maíz como sustrato microbiano para la obtención de xilanasas. Revista Científica la Universidad Autonoma de Coahuila 4. Available: Crossref
  6. Samanta, A.K., Senani, S., Kolte, A.P., Sridhar, M., Sampath, K.T., Jayapal, N. and Devi, A. (2012) Production and in vitro evaluation of xylooligosaccharides generated from corn cobs. Food Bioprod Process 90: 466-474. Crossref
  7. Pirota, R.D.P.B., Delabona, P.S. and Farinas, C.S. (2014) Simplification of the biomass to ethanol conversion process by using the whole medium of ilamentous fungi cultivated under solid-state fermentation. Bioenergy Res 7: 744-752. Crossref
  8. Nakazawa, H., Kawai, T., Ida, N., Shida, Y., Shioya, K., Kobayashi, Y., Okada, H., Tani, S., Sumitani, J., Kawaguchi, T., Morikawa, Y. and Ogasawara, W. (2016) A high performance Trichoderma reesei strain that reveals the importance of xylanase III in cellulosic biomass conversion. Enzyme Microb Technol 82: 89-95. Crossref
  9. Zhu, Z.Y., Zhao, L., Ge, X.R., Tang, Y.L., Chen, L.J., Pang, W. and Zhang, Y. (2015) Preparation, characterization and bioactivity of xylobiose and xylotriose from corncob xylan by xylanase. Eur Food Res Technol 241: 27-35. Crossref
  10. Silva, L.A.O., Terrasan, C.R.F. and Carmona, E.C. (2015) Purification and characterization of xylanases from Trichoderma inhamatum. Electron J Biotechnol 18: 307-313. Crossref
  11. Juturu, V. and Wu, J.C. (2012) Microbial xylanases: Engineering, production and industrial applications. Biotechnol Adv 30: 1219-1227. Crossref
  12. Almeida-Carvalho, E., Santos Góes, L.M., Uetanabaro, A.P.T., Paranhos da Silva, E.G., Rodrigues, L.B., Priminho-Pirovani, C. and da Costa, A.M. (2017) Thermoresistant xylanases from Trichoderma stromaticum : Application in bread making and manufacturing xylo-oligosaccharides. Food Chem 221: 1499-1506. Crossref
  13. Walia, A., Guleria, S., Mehta, P., Chauhan, A. and Prakash, J. (2017) Microbial xylanases and their industrial application in pulp and paper biobleaching: a review. 3 Biotech 7: 11. Crossref
  14. Yang, S., Huang, Z., Jiang, Z. and Li, L. (2008) Partition and purification of a thermostable xylanase produced by Paecilomyces thermophila in solid-state ermentation using aqueous two-phase systems. Process Biochem 43: 56-61. Crossref
  15. Garai, D. and Kumar, V. (2013) Aqueous two phase extraction of alkaline fungal xylanase in PEG/phosphate system: Optimization by Box-Behnken design approach. Biocatal Agric Biotechnol 2: 125-131. Crossref
  16. Nadar, S.S., Pawar, R.G. and Rathod, V.K. (2017) Recent advances in enzyme extraction strategies: A comprehensive review. Int J Biol Macromol 101: 931-957. Crossref
  17. Lee, K.M., Kalyani, D., Tiwari, M.K., Kim, T.S., Dhiman, S.S., Lee, J.K. and Kim, I.W. (2012) Enhanced enzymatic hydrolysis of rice straw by removal of phenolic compounds using a novel laccase from yeast Yarrowia lipolytica. Bioresour Technol 123: 636-645. Crossref
  18. Ruiz-Ruiz, F., Benavides, J., Aguilar, O. and Rito- Palomares, M. (2012) Aqueous two-phase affinity partitioning systems: Current applications and trends. J Chromatogr A 1244: 1-13. Crossref
  19. González-González, M., Mayolo-Deloisa, K., Rito-Palomares, M. and Winkler, R. (2011) Colorimetric protein quantification in aqueous two-phase systems. Process Biochem 46: 413-417. Crossref
  20. Loureiro, D.B., Romanini, D. and Tubio, G. (2016) Structural and functional analysis of Aspergillus niger xylanase to be employed in polyethylenglycol/salt aqueous two-phase extraction. Biocatal Agric Biotechnol 5: 204-210. Crossref
  21. Mayolo-Deloisa, K., Trejo-Hernández, M.D.R. and Rito-Palomares, M. (2009) Recovery of laccase from the residual compost of Agaricus bisporus in aqueous two-phase systems. Process Biochem 44: 435-439. Crossref
  22. Yasinok, A.E., Biran, S., Kocabas, A. and Bakir, U. (2010) Xylanase from a soil isolate, Bacillus pumilus: Gene isolation, enzyme production, purification, characterization and one-step separation by aqueous-two-phase system. World J Microbiol Biotechnol 26: 1641-1652. Crossref
  23. Miller, G.L. (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31: 426-428. Crossref
  24. Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254. Crossref
  25. Simões, M.L.G., Tauk-Tornisielo, S.M. and Tapia, D.M. (2009) Screening of culture condition for xylanase production by filamentous fungi. Afr J Biotechnol 8: 6317-6326. Crossref
  26. Chapla, D., Pandit, P. and Shah, A. (2012) Production of xylooligosaccharides from corncob xylan by fungal xylanase and their utilization by probiotics. Bioresour Technol 115: 215-221. Crossref
  27. Grujic, M., Dojnov, B., Potocnik, I., Duduk, B. and Vujcic, Z. (2015) Spent mushroom compost as substrate for the production of industrially important hydrolytic enzymes by fungi Trichoderma spp . and Aspergillus niger in solid state fermentation. Int Biodeterior Biodegradation 104: 290-298. Crossref
  28. Jun, H., Kieselbach, T. and Jönsson, L.J. (2011) Enzyme production by filamentous fungi: analysis of the secretome of Trichoderma reesei grown on unconventional carbon source. Microb Cell Fact 10: 68. Crossref
  29. Duarte, A.W.F., Lopes, A.M., Molino, J.V.D., Pessoa, A. and Sette, L.D. (2015) Liquid-liquid extraction of lipase produced by psychrotrophic yeast Leucosporidium scottii L117 using aqueous two-phase systems. Sep Purif Technol 156: 215-225. Crossref
  30. Sánchez-Trasviña, C., González-Valdez, J., Mayolo-Deloisa, K. and Rito-Palomares, M. (2015) Impact of aqueous two-phase system design parameters upon the in situ refolding and recovery of invertase. J Chem Technol Biotechnol 90: 1765-1772. Crossref